Assessing the Clinical Significance of Monocyte Chemoattractant Protein 1 and Protein Oxidation Products in Patients with Chronic Periodontitis
DOI:
https://doi.org/10.31351/vol35iss2pp125-135Keywords:
Monocyte chemoattractant protein‐1, Nitrotyrosine, Oxidative stress, Periodontitis, Protein carbonylAbstract
An essential chemokine called monocyte chemoattractant protein-1 (MCP-1) is in charge of initiating, controlling, and mobilizing monocytes to the sites of active severe periodontal inflammation. The purpose of the current study is to assess the serum levels of MCP-1 and protein oxidation products in chronic periodontitis patients, namely Advanced Oxidation Protein Products (AOPP), 3-Nitrotyrosine (3-NT), and Protein Carbonyl (PC). Evaluation of potential relationships between MCP-1 levels and the byproducts of protein oxidation. In the current investigation, 45 patients with severe chronic periodontitis (diseased) and 14 healthy individuals took part. Enzyme-linked immunosorbent assay was used to measure the amounts of MCP-1, AOPP, 3-NT, and PC in samples obtained from each participant. Using R statistical software, tests of correlation and linear regression were carried out. Serum levels of MCP-1, AOPP, 3-NT, and PC were considerably greater in the periodontitis group than in the control group among those with periodontitis. Protein oxidation metrics and MCP-1 exhibited a positive connection. It is indicated that elevated levels of MCP-1, AOPP, 3-NT, and PC could be a possible biomarker for periodontal disorders and that these levels may play a significant role during periodontal inflammation.
How to Cite
Publication Dates
Received
Revised
Accepted
Published Online First
References
Rodríguez-Archilla A, Mohamed-El-Founti N. Association of periodontitis with vitamin D and calcium levels: a meta-analysis. Iberoamerican Journal of Medicine. 2023;5(1):36-45.
Pradeep AR, Daisy H, Hadge P. Gingival crevicular fluid levels of monocyte chemoattractant protein-1 in periodontal health and disease. Archives of Oral Biology. 2009 May 1;54(5):503-9.
Trivedi S, Lal N, Mahdi AA, Mittal M, Singh B, Pandey S. Evaluation of antioxidant enzymes activity and malondialdehyde levels in patients with chronic periodontitis and diabetes mellitus. Journal of periodontology. 2014 May;85(5):713-20.
Nishikawa T, Naruse K, Kobayashi Y, Miyajima S, Mizutani M, Kikuchi T, Soboku K, Nakamura N, Sokabe A, Tosaki T, Hata M. Involvement of nitrosative stress in experimental periodontitis in diabetic rats. Journal of clinical periodontology. 2012 Apr;39(4):342-9.
Baltacioglu E, Sukuroglu E. Protein carbonyl levels in serum, saliva and gingival crevicular fluid in patients with chronic and aggressive periodontitis. The Saudi dental journal. 2019 Jan 1;31(1):23-30.
Zhou C, Zhang Y, Chen J, Mei C, Xiong F, Shi W, Zhou W, Liu X, Sun S, Tian J, Ye Z. Association between serum advanced oxidation protein products and mortality risk in maintenance hemodialysis patients. Journal of Translational Medicine. 2021 Jun 30;19(1):284.
Wang F, Yuan Q, Chen F, Pang J, Pan C, Xu F, Chen Y. Fundamental mechanisms of the cell death caused by nitrosative stress. Frontiers in Cell and Developmental Biology. 2021 Sep 20;9:742483.
Dalle‐Donne I, Aldini G, Carini M, Colombo R, Rossi R, Milzani A. Protein carbonylation, cellular dysfunction, and disease progression. Journal of cellular and molecular medicine. 2006 Apr;10(2):389-406.
Baltacıoğlu E, Akalın FA, Alver A, Değer O, Karabulut E. Protein carbonyl levels in serum and gingival crevicular fluid in patients with chronic periodontitis. Archives of oral biology. 2008 Aug 1;53(8):716-22.
Androsz-Kowalska O, Jankowski K, Rymarczyk Z, Kowalski J, Pruszczyk P, Górska R. Correlation between clinical parameters of periodontal disease and mean platelet volume in patients with coronary artery disease: a pilot study. Kardiologia Polska (Polish Heart Journal). 2013;71(6):600-5.
Zhu H, Lin X, Zheng P, Chen H. Inflammatory cytokine levels in patients with periodontitis and/or coronary heart disease. International Journal of Clinical and Experimental Pathology. 2015;8(2):2214.
Wang L, Yang L, Gao L, Gao TW, Li W, Liu YF. A functional promoter polymorphism in monocyte chemoattractant protein‐1 is associated with psoriasis. International Journal of Immunogenetics. 2008 Feb;35(1):45-9
Radhakrishnan P, Srikanth P, Seshadri KG, Barani R, Samanta M. Serum monocyte chemoattractant protein-1 is a biomarker in patients with diabetes and periodontitis. Indian journal of endocrinology and metabolism. 2014 Jul;18(4):505.
Silva TA, Garlet GP, Fukada SY, Silva JS, Cunha FD. Chemokines in oral inflammatory diseases: apical periodontitis and periodontal disease. Journal of dental research. 2007 Apr;86(4):306-19.
Hendek MK, Erdemir EO, Kisa U. Advanced oxidation protein products and monocyte chemoattractant protein-1 in periodontal disease. Turk J Clin Lab. 2018;9(1):1-6.
Pouliou C, Piperi C. Advances of Oxidative Stress Impact in Periodontitis: Biomarkers and Effective Targeting Options. Current Medicinal Chemistry. 2024 Nov;31(38):6187-203.
Shang J, Liu H, Zheng Y, Zhang Z. Role of oxidative stress in the relationship between periodontitis and systemic diseases. Frontiers in Physiology. 2023;14.
Chu X, Sun Z, Baek DS, Li W, Mellors JW, Shapiro SD, Dimitrov DS. Human antibody domains and fragments targeting neutrophil elastase as candidate therapeutics for cancer and inflammation-related diseases. International Journal of Molecular Sciences. 2021 Oct 15;22(20):11136.
Sui L, Wang J, Xiao Z, Yang Y, Yang Z, Ai K. ROS-scavenging nanomaterials to treat periodontitis. Frontiers in Chemistry. 2020 Nov 4;8:595530.
Wang XS, Zhang ZR, Zhang MM, Sun MX, Wang WW, Xie CL. Neuroprotective properties of curcumin in toxin-base animal models of Parkinson’s disease: a systematic experiment literatures review. BMC complementary and alternative medicine. 2017 Dec;17(1):1-0.
Zhang Z, Zheng Y, Bian X, Wang M, Chou J, Liu H, Wang Z. Identification of Key Genes and Pathways Associated with Oxidative Stress in Periodontitis. Oxidative Medicine and Cellular Longevity. 2022;2022.
Almerich-Silla JM, Pastor S, Serrano F, Puig-Silla M, Dasí F. Oxidative stress parameters in saliva and its association with periodontal disease and types of bacteria. Disease markers. 2015 Oct 1;2015.
De Angelis P, Gasparini G, Manicone PF, Passarelli PC, Azzolino D, Rella E, De Rosa G, Papi P, Pompa G, De Angelis S, Grassi R. The effect of an optimized diet as an adjunct to non-surgical periodontal therapy in subjects with periodontitis: a prospective study. InHealthcare 2022 Mar 21 (Vol. 10, No. 3, p. 583).
Kelly FJ, Mudway IS. Protein oxidation at the air-lung interface. Amino acids. 2003 Dec;25:375-96.
Dharuman S, Kumar SA, Balaji SK, Vishwanath U, Parameshwari RP, Santhanakrishnan M. Evaluation of levels of advanced oxidative protein products in patients with polycystic ovary syndrome with and without chronic periodontitis: A cross-sectional study. International Journal of Fertility & Sterility. 2022 Jan;16(1):55.
Bullon P, Newman HN, Battino M. Obesity, diabetes mellitus, atherosclerosis and chronic periodontitis: a shared pathology via oxidative stress and mitochondrial dysfunction?. Periodontology 2000. 2014 Feb;64(1):139-53.
Oztürk OH, Cetin AY, Ozdem SS, Uysal NU, Kayişli UA, Sentürk UK, Yeşilkaya AK. Plasma levels of nitrites, PGF1alpha, and nitrotyrosine in LPS-treated rats: functional and histochemical implications in aorta. Journal of physiology and biochemistry. 2006 Mar 1;62(1):27-34.
Sugiura H, Liu X, Ichikawa T, Ichinose M, Rennard SI. 3-Nitrotyrosine inhibits fibroblast-mediated collagen gel contraction and chemotaxis. European Respiratory Journal. 2009 Dec 1;34(6):1452-60.
Ichinose M, Sugiura H, Yamagata S, Koarai A, Shirato K. Increase in reactive nitrogen species production in chronic obstructive pulmonary disease airways. American journal of respiratory and critical care medicine. 2000 Aug 1;162(2):701-6.
Fu Z, Zhang J, Zhang Y. Role of molecular hydrogen in ageing and ageing-related diseases. Oxidative Medicine and Cellular Longevity. 2022 Mar 18; 2022.
Matsuo K, Hosoda K, Tanaka J, Yamamoto Y, Imahori T, Nakai T, Irino Y, Shinohara M, Sasayama T, Kohmura E. Geranylgeranylacetone attenuates cerebral ischemia–reperfusion injury in rats through the augmentation of HSP 27 phosphorylation: a preliminary study. BMC neuroscience. 2021 Dec;22(1):1-1.
Caliri AW, Tommasi S, Besaratinia A. Relationships among smoking, oxidative stress, inflammation, macromolecular damage, and cancer. Mutation Research/Reviews in Mutation Research. 2021 Jan 1;787:108365.
Sologova SS, Zavadskiy SP, Mokhosoev IM, Moldogazieva NT. Short linear motifs orchestrate functioning of human proteins during embryonic development, redox regulation, and cancer. Metabolites. 2022 May 21;12(5):464.
Baltacıoğlu E, Kehribar MA, Yuva P, Alver A, Atagün ÖS, Karabulut E, Akalın FA. Total oxidant status and bone resorption biomarkers in serum and gingival crevicular fluid of patients with periodontitis. Journal of periodontology. 2014 Feb;85(2):317-26.
Pradeep AR, Ramchandraprasad MV, Bajaj P, Rao NS, Agarwal E. Protein carbonyl: An oxidative stress marker in gingival crevicular fluid in healthy, gingivitis, and chronic periodontitis subjects. Contemporary clinical dentistry. 2013 Jan;4(1):27.
Baraibar MA, Ladouce R, Friguet B. Proteomic quantification and identification of carbonylated proteins upon oxidative stress and during cellular aging. Journal of proteomics. 2013 Oct 30;92:63-70.
Munteanu IR, Luca RE, Mateas M, Darawsha LD, Boia S, Boia ER, Todea CD. The Efficiency of Photodynamic Therapy in the Bacterial Decontamination of Periodontal Pockets and Its Impact on the Patient. Diagnostics. 2022 Dec 2;12(12):3026.
Liu X, Hou Y, Yang M, Xin X, Deng Y, Fu R, Xiang X, Cao N, Liu X, Yu W, Yang B, Zhou Y. N‐Acetyl‐L‐Cysteine‐Derived Carbonized Polymer Dots with ROS Scavenging via Keap1‐Nrf2 Pathway Regulate Alveolar Bone Homeostasis in Periodontitis. Advanced Healthcare Materials. 2023 Jun 6:2300890.
Willmann C, Mata X, Hanghoej K, Tonasso L, Tisseyre L, Jeziorski C, Cabot E, Chevet P, Crubézy E, Orlando L, Esclassan R. Oral health status in historic population: Macroscopic and metagenomic evidence. PLoS One. 2018 May 16; 13(5):e0196482.
Chu X, Sun Z, Baek DS, Li W, Mellors JW, Shapiro SD, Dimitrov DS. Human antibody domains and fragments targeting neutrophil elastase as candidate therapeutics for cancer and inflammation-related diseases. International Journal of Molecular Sciences. 2021 Jan;22(20):11136.
Wang Y, Andrukhov O, Rausch-Fan X. Oxidative stress and antioxidant system in periodontitis. Frontiers in physiology. 2017 Nov 13;8:910.
Sui L, Wang J, Xiao Z, Yang Y, Yang Z, Ai K. ROS-scavenging nanomaterials to treat periodontitis. Frontiers in chemistry. 2020 Nov 4;8:595530.
Monmeesil P, Fungfuang W, Tulayakul P, Pongchairerk U. The effects of astaxanthin on liver histopathology and expression of superoxide dismutase in rat aflatoxicosis. Journal of Veterinary Medical Science. 2019;81(8):1162-72.
Downloads
Published
License
Copyright (c) 2026 Iraqi Journal of Pharmaceutical Sciences
This work is licensed under a Creative Commons Attribution 4.0 International License.
